Journal: Brain and Behavior

Article Title: Biological effects of exposure to 2650 MHz electromagnetic radiation on the behavior, learning, and memory of mice

doi: 10.1002/brb3.3004

Figure Lengend Snippet: The effect of 2650 MHz‐EMR on hippocampal expression of BDNF and GABA ( n = 4). (A) Western blot reaction protein bands of BDNF and GABA from hippocampal tissue. Tubulin protein bands were used as internal controls for each gel. (B) BDNF; (C) GABA. The band quantification is expressed as the mean ± SD relative to the relative intensity of tubulin.

Article Snippet: After the 5% skim milk powder was blocked for 4 h, primary anti‐BDNF (Boster Biological Technology Co. Ltd, 37 KD), GABA (Abcam, 50 KD), and Tubulin (Boster Biological Technology Co. Ltd, 55 KD) antibodies were added at a ratio of 1:50, 1 μg/mL, and 1:500, respectively, and incubated overnight at 4°C.

Techniques: Expressing, Western Blot

Journal: iScience

Article Title: Derived myeloid lineage induced pluripotent stem as a platform to study human C-C chemokine receptor type 5Δ32 homozygotes

doi: 10.1016/j.isci.2023.108331

Figure Lengend Snippet:

Article Snippet: After 24 hours of recovery in Essential 8TM Medium (Gibco), in order to drive cells into mesoderm progenitor differentiation, cells were allowed to grow in chemically defined differentiation medium (MDM) containing Iscove’s modified Dulbecco’s medium (IMDM, Invitrogen) supplemented with Ham's F-12 Nutrient Mix, GlutaMAXTM supplement (Invitrogen) at 1:1 ratio, Albucult (recombinant human - rh Albumin) (5 mg/ml; Novozymes Delta), a-Monothioglycerol (a-MTG) (3.9 ml per 100 ml; Sigma-Aldrich), Protein-free hybridoma mixture II (PFHMII) (5%; Invitrogen), Ascorbic acid 2 phosphate (50 μg/ml; Sigma-Aldrich), Penicillin/streptomycin (50 U Pen G/50 mg streptomycin sulfate; Invitrogen), Insulin Transferrin Selenium-X Supplement (Invitrogen).

Techniques: Virus, Recombinant, Modification, Purification, Blocking Assay, Control, Library Quantification, Luminex, Pore Size, Amplification, Software